Critical Parameters for Successful siRNA Experiments
Prior to beginning siRNA experiments multiple parameters associated with experimental design need to be optimized. In particular, did you for instance know
- How to use our optimized protocols for siRNA experiments?
- It is essential to choose appropriate controls?
- You should test different siRNA concentrations to find out the optimal amount for your target gene in your cell type, as the knockdown level is
- cell-type specific, i.e. you might see different results with one and the same siRNA if you transfer it in different cell types?
- target-specific, i.e. different targets in one and the same cell type may require different conditions
- The kinetics of down-regulation vary depending on your target gene and on the analysis level?
- It is important to identify a suitable and robust readout assay for your particular application (i.e. mRNA, protein or phenotypic analysis)
