Citation Details
Long-term transgene expression in mouse neural progenitor cells modified with PhiC31 integrase
| Authors | Keravala A, Ormerod BK, Palmer TD, Calos MP |
| In | J Neurosci Methods (2008) 173(2): 299-305 |
| Genes of Interest | GFP, Luciferase |
| Cells used in publication | Neural stem cell (NSC), mouse Mouse neural stem cells (NSC) Stem Cells Primary Cells Species: mouse Tissue Origin: brain |
| Substrate | Plasmid (general) |
| Applications | Stable Transfection Co-Transfection |
| Topics | Gene therapy Luciferase |
| Research Areas | Neurobiology Stem Cells |
| Reporter Genes | eGFP Firefly Luciferase |
Research Field
Neurobiology, Stem cells, Gene Therapy
Nucleofection Experiments
Nucleofection of mNPCs with plasmid coding for PhiC31 Inegrase along with either a plasmid containing AttB CMV and GFP or a plasmid containing AttB CMV and Luciferase to generate targeted clones.
