Citation Details
Regulation of Self-renewal and Pluripotency by Sox2 in Human Embryonic Stem Cells
| Authors | Fong H, Hohenstein KA, Donovan PJ |
| In | Stem Cells (2008) 26(8): 1931-8 |
| Cells used in publication | Embryonic stem (ES) cell, human Human embryonic stem (ES) cells Stem Cells Primary Cells Species: human Tissue Origin: embryo |
| Substrate | Plasmid (general) siRNA |
| Applications | RNAi Co-Transfection |
| Topics | Cell morphology/cytoskeleton Differentiation of nucleofected cells Microscopy of nucleofected cells |
| Research Area | Stem Cells |
| Relevant Products | Nucleofector Device |
| Reporter Gene | maxGFP |
| Vector Backbone | pmaxGFP |
Nucleofection Experiments
Human embryonic stem cells (H1, H9 lines) were nucleofected with siRNAs against beta-2-microglobulin (beta2M), Nanog, Oct4 and Sox2. Three additional siRNAs targeting Sox2 were also used to verify the phenotype and eliminate the possibility of off-tartget effects. In initial experiments, each siRNA was co-transfected with pmaxGFP vector in a ratio of 5:1 to identify transfected cells and determine morphology.
