Citation Details
c-Myc degradation induced by DNA damage results in apoptosis of CHO cells
| Authors | Jiang MR, Li YC, Yang Y, Wu JR |
| In | Oncogene (2003) 22: 3252-3259 |
| Cells used in publication | UV41 (ATCC) Connective Tissue Cells Cell Lines Species: hamster Tissue Origin: ovarian |
| Substrate | Plasmid (general) |
| Topics | Apoptosis Diseases (e.g. HIV) Microscopy of nucleofected cells Signal transduction |
| Research Area | Cancer Research/Cell Biology |
| Reporter Gene | eGFP |
| Vector Backbone | pEGFP |
Research Field
Tripchlorolide (TC), a chemical purified from a Chinese herb and a potent antitumor agent, induces apoptosis in Chinese Hamster Ovary (CHO) cells, especially in a UV41-CHO cell line with a deficiency in a gene coding for a nucleotide excision repair protein. This links apoptosis after TC treatment with DNA damage. Concomitantly, strong degradation of the proto-oncogenic protein c-Myc is observed.
Nucleofection Experiments
UV41-CHO cells nucleofected with GFP tagged c-Myc and treated with TC showed a decreased amount of apoptotic cells, as scored by flow cytometry and counting of condensed nuclei. C-Myc degradation as a result of DNA damage is proposed to induce apoptosis.
