Details for FAQ article #9
«Is there a special recommendation on Human T cell enrichment before Nucleofection®?»
For Nucleofection® it is preferable to use Human T cell populations
enriched by magnetic separation. For example, MACS® Microbeads (Miltenyi) or by a rosetting method.
For magnetic separation we recommend negative selection or detaching beads after enrichment. Do not apply protocols using hypo-osmolar buffers.
This may lead to high cell mortality after Nucleofection.
Relations for this FAQ article
| Research Areas | Immunology / Hematology |
| Categories | Cell Prep. And Handling |
| Cells | T cell, human peripheral blood unstim. T cell, human stim. |
| Products |
