Details for FAQ article #11

«How can I increase cell viability of Dendritic cells after Nucleofection®?»

DNA amount and qualitiy are very critical for Nucleofection® of Dendritic Cells (recommended 0.5-1µg; maximal 2 µg). LPS (lipopolysaccharides) have a strong negative effect on cell viability. Please make sure that all components you use for dendritic cell culture and transfection, e.g. PBS, FCS and especially DNA, are LPS free. We additionally recommend purifying your
DNA by precipitating it twice with 20% PEG/2.5 M NaCl.
Viability is usually not an issue when working with mRNA or siRNA.