FAQ Database - Complete List

350 FAQ articles available.

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21. Do you have any helpful calculations or conversion information for siRNA?

22. Who is the distributor for my country?

23. Why do you have different Optimized Protocols for unstimulated and stimulated Human T cells?

24. Is it possible to use frozen primary CD34+ hematopoietic stem cells for Nucleofection?

25. Is it possible to transfect mRNA of tumor cells into antigen-presenting cells like B cells or dendritic cells with the Nucleofector Technology?

26. What are the critical steps for successful transfection of monocytic cell lines like THP-1, HL60 and U937?

27. Do you recommend a particular supplier for siRNA duplexes?

28. Can I use the protocol for MCF7 cells for other breast cancer cell lines?

29. When nucleofecting cancer cell lines, do I need to be concerned about passage number?

30. Why am I not able to detect my fluorescent label (e.g. FITC) after 24 or 48 hours post-Nucleofection?

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