FAQ Database - Complete List

350 FAQ articles available.

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11. I want to stimulate Human T cells post Nucleofection®. Are there any precautions to keep in mind?

12. Is it possible to get neomycin resistant clones after Nucleofection® with pmaxGFP®?

13. Where can I find the expiration dates for the kit and the Nucleofector solution?

14. Can I use the Nucleofector technology for cell fusion applications?

15. What is the shelf-life of the Nucleofector kits?

16. My Nucleofector solution was frozen. Is this a problem?

17. I have a GFP construct that I use and usually get anywhere from 50-80% GFP positive cells using FACS analysis while I get only 30-40% using a GFP-NFAT fusion protein driven by the same promoter. Do you have any suggestions?

18. I currently work with some adherent cell lines, such as 293, which are grown in DMEM media. I have noticed that the mortality seems to be high after Nucleofection. Do you have any recommendations?

19. Can I use the Nucleofector technology for RNAi applications? How do I start?

20. Can I order any of the kit components separately?

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